Shameik Moore

as Miles Morales (voice)

Hailee Steinfeld

as Gwen Stacy (voice)

Brian Tyree Henry

as Jeff Morales (voice)

Luna Lauren Velez

as Rio Morales (voice)

Jake Johnson

as Peter B. Parker (voice)

Oscar Isaac

as Miguel O'Hara (voice)

Jason Schwartzman

as Spot (voice)

Issa Rae

as Jessica Drew (voice)

Daniel Kaluuya

as Hobie Brown (voice)

Karan Soni

as Pavitr Prabhakar (voice)

Shea Whigham

as George Stacy (voice)

Greta Lee

as LYLA (voice)

Mahershala Ali

as Uncle Aaron (voice)

Amandla Stenberg

as Margo Kess: Spider-Byte (voice)

Jharrel Jerome

as Miles G. Morales (voice)

Andy Samberg

as Ben Reilly (voice)

Jack Quaid

as Peter Parker (voice)

Rachel Dratch

as Ms. Weber (voice)

Ziggy Marley

as Lenny (voice)

Jorma Taccone

as Adriano Tumino / '67 (voice)

J.K. Simmons

as J. Jonah Jameson (voice)

Donald Glover

as Aaron Davis

Elizabeth Perkins

as May / Quippy Spider-Person (voice)

Kathryn Hahn

as Doc Ock (voice)

Ayo Edebiri

as Glory (voice)

Nicole Delaney

as MJ (voice)

Antonina Lentini

as Betty (voice)

Atsuko Okatsuka

as Yuri (voice)

Peter Sohn

as Ganke (voice)

Melissa Sturm

as Mary Jane (voice)

Lorraine Velez

as Maria (voice)

Nic Novicki

as Lego Spider-Man (voice)

Taran Killam

as Web-Slinger (voice)

Metro Boomin

as Metro Spider-Man (voice)

Josh Keaton

as Spectacular Spider-Man (voice)

Sofia Barclay

as Malala Windsor: Spider-UK (voice)

Danielle Perez

as Charlotte Webber: Sun-Spider (voice)

Yuri Lowenthal

as Insomniac Spider-Man (voice)

Rita Rani Ahuja

as Additional Voices (voice)

Ismail Bashey

as Additional Voices (voice)

Oscar Camacho

as Additional Voices (voice)

Freddy Ferrari

as Additional Voices (voice)

Kerry Gutierrez

as Additional Voices (voice)

Kamal Khan

as Additional Voices (voice)

Angelo Sekou Kouyate

as Additional Voices (voice)

Andrew Leviton

as Additional Voices (voice)

David Michie

as Additional Voices (voice)

Sumit Naig

as Additional Voices (voice)

Juan Pacheco

as Additional Voices (voice)

Chrystee Pharris

as Additional Voices (voice)

Ben Pronsky

as Additional Voices (voice)

Al Rodrigo

as Additional Voices (voice)

Stan Sellers

as Additional Voices (voice)

Warren Sroka

as Additional Voices (voice)

Jasper Johannes Andrews

as Additional Voices (voice)

Gredel Berrios Calladine

as Additional Voices (voice)

Natalia Castellanos

as Additional Voices (voice)

Russell Tyre Francis

as Additional Voices (voice)

Deepti Gupta

as Additional Voices (voice)

Sohm Kapila

as Additional Voices (voice)

Pradnya Kuwadekar

as Additional Voices (voice)

Ashley London

as Additional Voices (voice)

Christopher Miller

as Additional Voices (voice)

Andrea Navedo

as Additional Voices (voice)

Lakshmi Patel

as Additional Voices (voice)

Jacqueline Pinol

as Additional Voices (voice)

Eliyas Qureshi

as Additional Voices (voice)

Lashana Rodriguez

as Additional Voices (voice)

Jaswant Dev Shrestha

as Additional Voices (voice)

Libby Thomas Dickey

as Additional Voices (voice)

Jason Linere-White

as Additional Voices (voice)

Sitara Attaie

as Additional Voices (voice)

Mayuri Bhandari

as Additional Voices (voice)

June Christopher

as Additional Voices (voice)

Michelle Jubilee Gonzalez

as Additional Voices (voice)

Marabina Jaimes

as Additional Voices (voice)

Rez Kempton

as Additional Voices (voice)

Lex Lang

as Additional Voices (voice)

Phil Lord

as Additional Voices (voice)

Richard Miro

as Additional Voices (voice)

Doug Nicholas

as Additional Voices (voice)

Shakira Ja'nai Paye

as Additional Voices (voice)

James Pirri

as Additional Voices (voice)

Marley Ralph

as Additional Voices (voice)

Michelle Ruff

as Additional Voices (voice)

Dennis Singletary

as Additional Voices (voice)

Amanda Troop

as Additional Voices (voice)

Ruth Zalduondo

as Additional Voices (voice)

Kimberly Bailey

as Additional Voices (voice)

Sanjay Chandani

as Additional Voices (voice)

Melanie Duke

as Additional Voices (voice)

Jorge R. Gutierrez

as Additional Voices (voice)

Miguel Jiron

as Additional Voices (voice)

Deepti Kingra-Mickelsen

as Additional Voices (voice)

Luisa Leschin

as Additional Voices (voice)

Caitlin McKenna

as Additional Voices (voice)

Andrew Morgado

as Additional Voices (voice)

Arthur Ortiz

as Additional Voices (voice)

Eliana A. Perez

as Additional Voices (voice)

Juan Pope

as Additional Voices (voice)

Mike Rianda

as Additional Voices (voice)

Erika Scopelli

as Additional Voices (voice)

Narender Sood

as Additional Voices (voice)

Cedric L. Williams

as Additional Voices (voice)

Kimiko Glenn

as Peni Parker (voice) (uncredited)

Peggy Lu

as Mrs. Chen